논문 상세보기

대한본초학회> 대한본초학회지(본초분과학회지)> 방풍류(防風類) 약재(藥材)의 신경세포보호효과 및 아세틸콜린에스터라제 저해 효과 비교

KCI등재

방풍류(防風類) 약재(藥材)의 신경세포보호효과 및 아세틸콜린에스터라제 저해 효과 비교

Comparative Study of Bang-poong (root of Saposhnikovia divaricata Schischkin) and Related Species on Neuroprotective and Acetylcholinesterase Inhibitory Effects

주인경 ( In Gyoung Ju ) , 이승민 ( Seungmin Lee ) , 최진규 ( Jin Gyu Choi ) , 오명숙 ( Myung Sook Oh )
  • : 대한본초학회
  • : 대한본초학회지(본초분과학회지) 34권5호
  • : 연속간행물
  • : 2019년 09월
  • : 29-37(9pages)

DOI


목차

Ⅰ. 서 론
Ⅱ. 재료 및 방법
Ⅲ. 결 과
Ⅳ. 고 찰
Ⅴ. 결 론
감사의 글
References

키워드 보기


초록 보기


						
Objectives : Bang-poong (Saposhnikovia divaricata ; SD) was traditionally used to treat inflammatory disorders. In this study, we aimed to investigate whether Bang-poong and related species including SD, Glehnia littoralis (GL), and Peucedanum japonicum (PJ) possess neuroprotective effects and acetylcholinesterase (AChE) inhibitory activities.
Methods : Roots of SD, GL and PJ were extracted with distilled water (DW) or 70% ethanol (EtOH). We assessed 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities of the extracts. To examine neuroprotective effects, we measured cell viability in PC12 or HT22 cells after treatment of the extracts with H2O2 or amyloid-beta (Aβ). To assess anti-neuroinflammatory effects, we measured the nitric oxide (NO) levels after treatment with the extracts and lipopolysaccharide (LPS) in BV2 microglial cells. In addition, we performed AChE inhibition assay to explore effects of the extracts on the cholinergic system.
Results : DW and EtOH extracts of SD, GL and PJ showed mild DPPH free radical scavenging activities. Also, DW extracts of GL and PJ showed protective effects against H2O2-induced toxicity in PC12 cells. In LPS-activated BV2 cells, EtOH extracts of SD, GL and PJ exerted inhibitory effects on NO production. Meanwhile, DW extracts of SD, GL and PJ inhibited the Aβ-induced cell death in HT22 cells. In addition, DW and EtOH extracts of GL exhibited remarkable inhibitory activities on AChE.
Conclusions : We demonstrated that SD, GL and PJ exert anti-oxidative, anti-neuroinflammatory and AChE inhibitory activities. These results indicate that SD, GL and PJ could be potential candidates for neurological disorders.

UCI(KEPA)

간행물정보

  • : 의약학분야  > 기초한의학
  • : KCI등재
  • :
  • : 격월
  • : 1229-1765
  • :
  • : 학술지
  • : 연속간행물
  • : 1986-2019
  • : 1557


저작권 안내

한국학술정보㈜의 모든 학술 자료는 각 학회 및 기관과 저작권 계약을 통해 제공하고 있습니다.

이에 본 자료를 상업적 이용, 무단 배포 등 불법적으로 이용할 시에는 저작권법 및 관계법령에 따른 책임을 질 수 있습니다.

발행기관 최신논문
| | | | 다운로드

1감초의 기원 및 교잡종 외내부형태 성상 비교연구

저자 : 김영식 ( Young-sik Kim ) , 박춘근 ( Chun-geon Park ) , 최고야 ( Goya Choi ) , 장재기 ( Jae-ki Chang ) , 이정훈 ( Jeong-hoon Lee ) , 주영승 ( Young-sung Ju )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 5호 발행 연도 : 2019 페이지 : pp. 1-12 (12 pages)

다운로드

(기관인증 필요)

초록보기

Objectives : The consumption of licorice is large in Korea, but domestic production is insufficient due to the lack of adaptability. This study aimed to provide a morphological basis for adding interspecific hybrid licorice with improved adaptability to pharmacopoeia.
Methods : This study was to establish identification criteria for the original plants, external and internal morphology of the authentic herbal medicines (Glycyrrhiza uralensis , G. glabra and G. inflata ), market products and artificially interspecific hybrid forms of licorice. For this purpose, previous studies were investigated and visual and histological observations were carried out. We focused on the internal morphology by microscopic observation for securing objectivity. Finally, we proposed the identification keys for precise classification of each part.
Results : 1) Original plant : Licorice species in the compendium were distinguished by the number of leaflets, presence of hair on the fruit, curvature and swelling of the fruit. 2) External morphology : Licorice species were distinguished by degree of powderiness, tearing gap, radial structure in the cross section and existence of protrusion of outer epidermis. 3) Internal morphology : Licorice species were distinguished by the degree of development of phloem fiber bundle, development of obliterated sieve, whether the secondary medullary ray are branched. In the case of interspecific hybrids, the characteristics of both species used for hybridization were mixed in all observation methods.
Conclusions : These results suggest that the interspecific crossbred licorice is suitable for the pharmacopoeial standard. Therefore, it can be applied as a herbal medicine through additional supplementary study.

2COI 염기서열 기반 백강잠 신속 감별용 SCAR marker 개발 - 백강잠 유전자 감별 -

저자 : 김욱진 ( Wook Jin Kim ) , 양선규 ( Sungyu Yang ) , 노푸름 ( Pureum Noh ) , 박인규 ( Inkyu Park ) , 최고야 ( Goya Choi ) , 송준호 ( Jun-ho Song ) , 문병철 ( Byeong Cheol Moon )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 5호 발행 연도 : 2019 페이지 : pp. 13-20 (8 pages)

다운로드

(기관인증 필요)

초록보기

Objectives : To ensure the safety, quality and pharmacological efficacy of Batryticatus Bombyx, it is important to discriminate with adulterants. In Korean Herbal Pharmacopoeias (KHP), the authentic species of Batryticatus Bombyx is defined only Bombyx mori. Therefore, the aim of this study is establishment of PCR assay method using the sequence characterized amplified region (SCAR) marker based on COI DNA barcode for discriminating six species related to Batryticatus Bombyx.
Methods : Seventeen samples of six species (Bombyx mori , Bombyx mandarina , Rhodinia fugax , Oberthueria caeca , Actias artemis, and Caligula japponica ) were collected from different habitate and nucleotide sequences of cytochrome c oxidase subunit I(COI) barcode regions were analyzed by Sanger sequencing methods. To develop SCAR-based PCR assay method, we designed species-specific primers based on COI sequence variabilities and verified those specificities using 17 samples of six species as well as commercial herbal medicines.
Results : In comparative multiple analysis of COI sequences, six species were distinguished by species-specific nucleotides at the species level. To develop rapid and reliable PCR assay method for genetic authentication of Batryticatus Bombyx, therefore, we designed species-specific SCAR primers based on these nucleotide sequences and confirmed those specificities. Using these SCAR primers, We also established simple conventional PCR assay method using these SCAR primers at the species level.
Conclusions : The comparative analysis of COI sequences and SCAR-based PCR assay methods represented equal results for distinguishing authentic Batryticatus Bombyx and adulterations at the species level. Therefore, our results are expected protecting adulteration of herbal medicine Batryticatus Bombyx.

3소청룡탕이 LPS로 유도된 폐손상 동물모델에 미치는 영향

저자 : 진보람 ( Bo-ram Jin ) , 최인영 ( In Young Choi ) , 황도영 ( Do Young Hwang ) , 함성호 ( Seong-ho Ham ) , 안효진 ( Hyo-jin An )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 5호 발행 연도 : 2019 페이지 : pp. 21-28 (8 pages)

다운로드

(기관인증 필요)

초록보기

Objectives : In present study, we investigated a therapeutic effect and optimum dose of Socheongryong-Tang (SCT) on LPS-induced lung inflammation rats model.
Methods : Male Sprague-Dawley rats (260 ± 10 g) were divided into 12 groups : Group 1 included the normal rats, and Group 2-12 were administrated LPS by intranasal injection to induce experimental lung inflammation. After 1 day of LPS administration, Group 3-9 were treated with SCT × 1/4, × 1/2, × 1, × 3, × 6, × 12 or × 18, respectively. Group 10-12 (positive control) were treated with dexamethasone 1 ㎎/㎏ or acetylcystein 1.5 ㎎/㎏ or diclofenac·sodium 0.4 ㎎/㎏, respectively. After sacrifice, bronchoalveolar lavage fluid (BALF) was isolated. The levels of IL-1β, TNF-α, mucin glycoprotein 5AC (MUG5AC) were measured in BALF using enzyme-linked immunosorbent assay (ELISA).
Results : LPS injected rats exhibited outstanding lung inflammation manifestations, including increased amount of total cells and neutrophil, and upregulated inflammatory cytokines level in BALF. However, the administration of SCT × 1/4, × 1/2 and × 1 decreased total cells and neutrophil, and suppressed the production of inflammatory cytokines, including IL-1β and TNF-α, and MUG5AC in BALF. Notably, inhibitory effect of SCT × 1/2 and × 1 on the level of TNF-α was markedly better than that of positive controls, dexamethasone and acetylcystein.
Conclusions : Taken together, these results suggest that SCT × 1/2 and × 1 has therapeutic effects on LPS-induced lung inflammation rats model.

4방풍류(防風類) 약재(藥材)의 신경세포보호효과 및 아세틸콜린에스터라제 저해 효과 비교

저자 : 주인경 ( In Gyoung Ju ) , 이승민 ( Seungmin Lee ) , 최진규 ( Jin Gyu Choi ) , 오명숙 ( Myung Sook Oh )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 5호 발행 연도 : 2019 페이지 : pp. 29-37 (9 pages)

다운로드

(기관인증 필요)

초록보기

Objectives : Bang-poong (Saposhnikovia divaricata ; SD) was traditionally used to treat inflammatory disorders. In this study, we aimed to investigate whether Bang-poong and related species including SD, Glehnia littoralis (GL), and Peucedanum japonicum (PJ) possess neuroprotective effects and acetylcholinesterase (AChE) inhibitory activities.
Methods : Roots of SD, GL and PJ were extracted with distilled water (DW) or 70% ethanol (EtOH). We assessed 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities of the extracts. To examine neuroprotective effects, we measured cell viability in PC12 or HT22 cells after treatment of the extracts with H2O2 or amyloid-beta (Aβ). To assess anti-neuroinflammatory effects, we measured the nitric oxide (NO) levels after treatment with the extracts and lipopolysaccharide (LPS) in BV2 microglial cells. In addition, we performed AChE inhibition assay to explore effects of the extracts on the cholinergic system.
Results : DW and EtOH extracts of SD, GL and PJ showed mild DPPH free radical scavenging activities. Also, DW extracts of GL and PJ showed protective effects against H2O2-induced toxicity in PC12 cells. In LPS-activated BV2 cells, EtOH extracts of SD, GL and PJ exerted inhibitory effects on NO production. Meanwhile, DW extracts of SD, GL and PJ inhibited the Aβ-induced cell death in HT22 cells. In addition, DW and EtOH extracts of GL exhibited remarkable inhibitory activities on AChE.
Conclusions : We demonstrated that SD, GL and PJ exert anti-oxidative, anti-neuroinflammatory and AChE inhibitory activities. These results indicate that SD, GL and PJ could be potential candidates for neurological disorders.

5열수추출 과정에서 삽주, 백출(큰꽃삽주), 북창출 배합이 감초 성분의 추출률에 미치는 영향

저자 : 김정훈 ( Jung-hoon Kim )

발행기관 : 대한본초학회 간행물 : 대한본초학회지(본초분과학회지) 34권 5호 발행 연도 : 2019 페이지 : pp. 39-47 (9 pages)

다운로드

(기관인증 필요)

초록보기

Objectives : When herbal medicines are extracted together, they may interact with each other, leading to change of chemical characteristics. This study aimed to evaluate the influence of Atractylodes rhizomes (Atractylodes japonica, A. macrocephala , and A. chinensis ) on the chemical features of the roots and rhizomes of Glycyrrhiza uralensis , which is are commonly combined with herbal medicines in many herbal formulae, when they are co-decocted.
Methods : Liquiritin apioside, liquiritin, ononin, and glycyrrhizin levels of G. uralensis in hot-water extracts prepared by the combination of Atractylodes rhizomes with various weight ratios (G. uralensis : Atractylodes rhizomes = 10:0, 10:5, 10:10, and 10:20) and extraction times (60, 90, and 120 min) were quantified using a HPLC-diode array detector and compared by statistical analysis.
Results : The concentrations of liquiritin apioside, liquiritin, ononin, and glycyrrhizin from G. uralensis roots and rhizomes mostly reduced when co-extracted with Atractylodes rhizomes, and the addition of A. chinensis most reduced their contents between Atractylodes combination groups. A. japonica and A. macrocephala rhizomes also showed differences of liquiritin and glycyrrhizin levels at 10 g and 20 g groups of Atractylodes rhizomes. Extraction times also affected the concentrations of liquiritin, ononin, and glycyrrhizin mostly during 60 and 90 min.
Conclusions : Atractylodes rhizomes might alter the chemical characteristics of G. uralensis when these herbs are co-decocted. This study provides the understanding of the chemical interactions of herbal medicines during the extraction in hot water.

1
주제별 간행물
간행물명 수록권호

KCI등재

대한한의학원전학회지
32권 4호 ~ 32권 4호

KCI등재

동의신경정신과학회지
30권 3호 ~ 30권 3호

동서의학
44권 2호 ~ 44권 2호

KCI등재

대한본초학회지(본초분과학회지)
34권 5호 ~ 34권 5호

KCI등재

Korean Journal of Acupuncture
36권 3호 ~ 36권 3호

KCI등재

대한침구의학회지
36권 3호 ~ 36권 3호

KCI등재

대한한방부인과학회지
32권 3호 ~ 32권 3호

KCI등재

대한한의학원전학회지
32권 3호 ~ 32권 3호

KCI등재

대한본초학회지(본초분과학회지)
34권 4호 ~ 34권 4호

동국한의학연구소 논문집
10권 0호 ~ 11권 0호

KCI등재

동의신경정신과학회지
30권 2호 ~ 30권 2호

KCI등재

Korean Journal of Acupuncture
36권 2호 ~ 36권 2호

KCI등재

대한한방부인과학회지
32권 2호 ~ 32권 2호

KCI등재

대한침구의학회지
36권 2호 ~ 36권 2호

KCI등재

대한본초학회지(본초분과학회지)
34권 3호 ~ 34권 3호

KCI등재

대한한의학원전학회지
32권 2호 ~ 32권 2호

동서의학
44권 1호 ~ 44권 1호

KCI등재

Korean Journal of Acupuncture
36권 1호 ~ 36권 1호

KCI등재

대한본초학회지(본초분과학회지)
34권 2호 ~ 34권 2호

KCI등재

동의신경정신과학회지
30권 1호 ~ 30권 1호
발행기관 최신논문
자료제공: 네이버학술정보
발행기관 최신논문
자료제공: 네이버학술정보

내가 찾은 최근 검색어

최근 열람 자료

맞춤 논문

보관함

내 보관함
공유한 보관함

1:1문의

닫기