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한국미생물생명공학회(구 한국산업미생물학회)> 한국미생물·생명공학회지> Improvement of Anthocyanin Encapsulation Efficiency into Yeast Cell by Plasmolysis, Ethanol, and Anthocyanin Concentration Using Response Surface Methodology

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Improvement of Anthocyanin Encapsulation Efficiency into Yeast Cell by Plasmolysis, Ethanol, and Anthocyanin Concentration Using Response Surface Methodology

Lieu My Dong , Hoang Thi Thuy Hang , Nguyen Huyen Nguyet Tran , Dang Thi Kim Thuy
  • : 한국미생물생명공학회(구 한국산업미생물학회)
  • : 한국미생물·생명공학회지 48권3호
  • : 연속간행물
  • : 2020년 09월
  • : 267-275(9pages)

DOI


목차

Introduction
Material and Methods
Results and Discussion
Conclusion
Conflict of Interest
References

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초록 보기

Anthocyanins are antioxidant compounds susceptible to environmental factors. Anthocyanin encapsulation into yeast cells is a viable solution to overcome this problem. In this study, the optimal factors for anthocyanin encapsulation were investigated, including anthocyanin concentration, plasmolysis contraction agent, and ethanol concentration, and response surface methodology was evaluated, for the first time. Anthocyanin from Hibiscus sabdariffa L. flowers was encapsulated into Saccharomyces cerevisiae using plasmolysis contraction agent (B: 3%-20% w/v), ethanol concentration (C: 3%-20% v/v), and anthocyanin concentration (A: 0.15-0.45 g/ml). The encapsulation yield and anthocyanin loss rate were determined using a spectrometer (520 nm), and color stability evaluation of the capsules was performed at 80℃ for 30 min. The results of the study showed that these factors have a significant impact on the encapsulation of anthocyanin, in which ethanol agents have the highest encapsulation yield compared to other factors in the study. Statistical analysis shows that the independent variables (A, B, C), their squares (A2, B2, C2), and the interaction between B and C have a significant effect on the encapsulation yield. The optimized factors were anthocyanin, 0.25 g/ml; NaCl, 9.5% (w/v); and ethanol, 11% (v/v) with an encapsulation yield of 36.56% ± 0.55% and anthocyanin loss rate of 15.15% ± 0.98%; This is consistent with the expected encapsulation yield of 35.46% and loss rate of 13.2%.

UCI(KEPA)

간행물정보

  • : 자연과학분야  > 생물
  • : KCI등재
  • : SCOPUS
  • : 계간
  • : 1598-642x
  • : 2234-7305
  • : 학술지
  • : 연속간행물
  • : 2002-2020
  • : 1112


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1Potential Application of Bacteriocin Produced from Lactic Acid Bacteria

저자 : Kaoutar El Issaoui , Nadia Skali Senhaji , Sanae Zinebi , Rajae Zahli , Imane Haoujar , Nadia Amajoud , Jamal Abrini , El Ouardy Khay

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 48권 3호 발행 연도 : 2020 페이지 : pp. 237-251 (15 pages)

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Lactic acid bacteria prevent the contamination of food products by inhibiting proliferation of pathogenic bacteria. This is done mainly by the production of lactic acid and antimicrobial peptides (AMPS) known as bacteriocins. The interest in these molecules resides in both their antimicrobial spectrum and safety for human health. The application of bacteriocins or producer strains has been considered to avoid the development of pathogenic bacteria, as most bacteriocins have significant inhibitory activity against food pathogens, such as Listeria monocytogenes. This article describes the classification, structure, mode of action, biosynthesis, and main applications of bacteriocins in different fields: agri-food, aquaculture, and medicine.

2A Comprehensive Study of SARS-CoV-2: From 2019-nCoV to COVID-19 Outbreak

저자 : Abdul Waris , Muhammad Ali , Atta Ullah Khan , Asmat Ali , Abdul Baset

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 48권 3호 발행 연도 : 2020 페이지 : pp. 252-266 (15 pages)

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The coronavirus disease 2019 (COVID-19) is a highly contagious pneumonia that has spread throughout the world. It is caused by a novel, single stranded RNA virus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Genetic analysis revealed that, phylogenetically, the SARS-CoV-2 is related to severe acute respiratory syndrome-like viruses seen in bats. Because of this, bats are considered as a possible primary reservoir. The World Health Organization has declared the COVID-19 outbreak as a pandemic. As of May 27, 2020, more than 5,406,282 confirmed cases, and 343,562 confirmed deaths have been reported worldwide. Currently, there are no approved vaccines or antiviral drugs available against COVID-19. Newly developed vaccines are in the first stage of clinical trials, and it may take a few months to a few years for their commercialization. At present, remdesivir and chloroquine are the promising drugs for treating COVID-19 patients. In this review, we summarize the diversity, genetic variations, primary reservoirs, epidemiology, clinical manifestations, pathogenesis, diagnosis, treatment strategies, and future prospects with respect to controlling the spread of COVID-19.

3Improvement of Anthocyanin Encapsulation Efficiency into Yeast Cell by Plasmolysis, Ethanol, and Anthocyanin Concentration Using Response Surface Methodology

저자 : Lieu My Dong , Hoang Thi Thuy Hang , Nguyen Huyen Nguyet Tran , Dang Thi Kim Thuy

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 48권 3호 발행 연도 : 2020 페이지 : pp. 267-275 (9 pages)

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Anthocyanins are antioxidant compounds susceptible to environmental factors. Anthocyanin encapsulation into yeast cells is a viable solution to overcome this problem. In this study, the optimal factors for anthocyanin encapsulation were investigated, including anthocyanin concentration, plasmolysis contraction agent, and ethanol concentration, and response surface methodology was evaluated, for the first time. Anthocyanin from Hibiscus sabdariffa L. flowers was encapsulated into Saccharomyces cerevisiae using plasmolysis contraction agent (B: 3%-20% w/v), ethanol concentration (C: 3%-20% v/v), and anthocyanin concentration (A: 0.15-0.45 g/ml). The encapsulation yield and anthocyanin loss rate were determined using a spectrometer (520 nm), and color stability evaluation of the capsules was performed at 80℃ for 30 min. The results of the study showed that these factors have a significant impact on the encapsulation of anthocyanin, in which ethanol agents have the highest encapsulation yield compared to other factors in the study. Statistical analysis shows that the independent variables (A, B, C), their squares (A2, B2, C2), and the interaction between B and C have a significant effect on the encapsulation yield. The optimized factors were anthocyanin, 0.25 g/ml; NaCl, 9.5% (w/v); and ethanol, 11% (v/v) with an encapsulation yield of 36.56% ± 0.55% and anthocyanin loss rate of 15.15% ± 0.98%; This is consistent with the expected encapsulation yield of 35.46% and loss rate of 13.2%.

4Probiotic Potential of Pediococcus acidilactici and Enterococcus faecium Isolated from Indigenous Yogurt and Raw Goat Milk

저자 : Shovon Lal Sarkar , Md Iqbal Hossain , Sharmin Akter Monika , Santonu Kumar Sanyal , Pravas Chandra Roy , Md Anwar Hossain , Iqbal Kabir Jahid

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 48권 3호 발행 연도 : 2020 페이지 : pp. 276-286 (11 pages)

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Probiotics are live microorganisms that, when administered in adequate amounts, confer health benefits to the host. This study was conducted for the isolation of potential lactic acid bacteria (LAB) with probiotic properties from goat milk and yogurt. Several tests were conducted in vitro using the standard procedures for evaluating the inhibitory spectra of LAB against pathogenic bacteria; tolerance to NaCl, bile salt, and phenol; hemolytic, milk coagulation, and bile salt hydrolase activities; gastrointestinal transit tolerance; adhesion properties; and antibiotic susceptibility. Among 40 LAB strains screened according to culture characteristics, five isolates exhibited antagonistic properties. Three were identified as Pediococcus acidilactici, and two were identified as Enterococcus faecium, exploiting 16S rRNA gene sequencing. All the isolates succeeded in the gastrointestinal transit tolerance assay and successively colonized mucosal epithelial cells. Based on the results of these in vitro assays, both P. acidilactici and E. faecium can be considered as potential probiotic candidates.

5Properties of β-Galactosidase from Lactobacillus zymae GU240, an Isolate from Kimchi, and Its Gene Cloning

저자 : Huong Giang Le , Zhuang Yao , Jeong A Kim , Se Jin Lee , Yu Meng , Ji Yeong Park , Jeong Hwan Kim

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 48권 3호 발행 연도 : 2020 페이지 : pp. 287-295 (9 pages)

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Lactobacillus zymae GU240 was previously isolated from Kimchi, a Korean fermented vegetable, as a strong GABA producer. The strain showed β-galactosidase (β-Gal) activity on MRS agar plates with X-gal. When growth and β-Gal activities of GU240 were measured using MRS (glucose, 2%, w/v) and MRSL (lactose, 2%, w/v) broths, cells were found to grow slowly in MRSL, and the β-Gal activity (36 units at 4 h) was lower than that of cells grown in MRS (94 units at 16 h). The highest OD600 value of the culture in MRS was 1.6 at 24 h at 37℃, whereas that of the culture in MRSL was 0.6 at 16 h. β-Gal activity of the culture in MRS reached the maximum (95.6 u/ml) at 16 h, decreased thereafter, and was not detected at 48 h. β-Gal activity for culture in MRSL reached its highest (36 u/ml) at 4 h and decreased gradually, but some activity (11.05 u/ ml) still remained at 72 h. The structural gene encoding β-Gal in L. zymae GU240 was cloned as a 3.1 kb fragment, and DNA sequencing confirmed the presence of complete lacLM genes. lacLM genes from L. zymae GU240 showed 98-99% homologies in nucleotide sequences with other lacLM genes from L. brevis. Reverse transcription (RT)-PCR confirmed the operon structure of lacLM. The results indicated that L. zymae GU240 might be in the process of losing the ability to grow rapidly on lactose-containing medium, such as milk, due to adaptations to plant environments, including kimchi.

6Effects of Glycerol and Shikimic Acid on Rapamycin Production in Streptomyces rapamycinicus

저자 : Huyen Thi Huong La , Thao Kim Nu Nguyen , Hang Thuy Dinh , Quyen Minh Huynh Nguyen , Minh Hong Nguyen

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 48권 3호 발행 연도 : 2020 페이지 : pp. 296-302 (7 pages)

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Rapamycin, derived from Streptomyces rapamycinicus, is an important bioactive compound having a therapeutic value in managing Parkinson's disease, rheumatoid arthritis, cancer, and AIDS. Because of its pharmaceutical activity, studies over the past decade have focused on the biosynthesis of rapamycin to enhance its yield. In this study, the effect of rapG on rapamycin production was investigated. The rapG expression vector was constructed by utilizing the integration vector pSET152 under the control of the erythromycin resistance gene (ermE*), a strong constitutive promoter. The rapamycin yield of wild type (WT) and WT/ rapG overexpression mutant strains, under fermentation conditions, was analyzed by high-performance liquid chromatography (HPLC). Our results revealed that overexpression of rapG increased rapamycin production by approximately 4.9-fold (211.4 mg/l) in MD1 containing 15 g/l of glycerol, compared to that of the WT strain. It was also found that Illicium verum powder (10 g/l), containing shikimic acid, enhanced rapamycin production in both WT and WT/rapG strains. Moreover, the amount of rapamycin produced by the WT/rapG strain was statistically higher than that produced by the WT strain. In conclusion, the addition 15 g/l glycerol and 15 g/l I. verum powder produced the optimal conditions for rapamycin production by WT and WT/rapG strains.

7유산균으로 발효한 침향공진단으로부터 분리한 Nodakenetin의 Neuraminidase 활성 억제 효능

저자 : 서지현 ( Ji Hyun Seo ) , 박동준 ( Dong Jun Park ) , 이소영 ( So Young Lee ) , 조호성 ( Ho Song Cho ) , 진무현 ( Mu Hyun Jin )

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 48권 3호 발행 연도 : 2020 페이지 : pp. 303-309 (7 pages)

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대표적인 한방 보약 처방인 원방공진단을 재해석한 침향공진단 (당귀, 녹용, 산수유, 및 침향의 혼합추출물)을 유산균으로 발효하고, 침향공진단 성분 중 발효를 통해 증가하는 성분을 분리, 정제하고 nodakenetin임을 동정하였다. 발효전 침향공진단(침향공진단 농축액 1% 함유 MRS 배지, unfermented Gongjin-dan, GD) 및 발효후 침향공진단(침향공진단 발효액, fermented Gongjin-dan, FGD)에서의 nodakenetin 함량 분석 결과, 각각 6 μg/ml과 70 μg/ml으로 발효를 통해 nodakenetin이 약 10배 이상 증가하였다. 한편, 고서에 전해지는 공진단의 면역력 강화 효능에 근거하여, GD 및 FGD의 인플루엔자 바이러스 증식 억제 효능을 확인하고자 Neuraminidase (NA) 활성 평가법(NA activity assay)을 실시하였다. 실험 결과, GD는 NA 활성을 억제하지 못하였으나, FGD는 농도의존적으로 NA 활성을 억제하였으며 500 μg/ml에서 대조군 대비 약 92%의 억제율을 보였다. 또한, 발효를 통해 증가한 침향공진단의 성분인 nodakenetin과 그 배당체인 nodakenin에 대한 NA 활성 평가 결과, nodakenin은 NA 활성을 거의 억제하지 못하였으나, nodakenetin은 농도의존적으로 NA 활성을 억제하였으며 250 μg/ml에서 대조군 대비 약 68%의 억제율을 보였다. 이상의 결과들을 종합하여, 유산균 발효를 통해 침향공진단 내에 미량 존재하던 nodakenetin이 nodakenin의 가수분해로 인해 증가하였으며, NA 활성 억제 성분인 nodakenetin이 증가함으로 인해 FGD도 높은 NA 활성 억제 효능을 보였다고 판단할 수 있었다.

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본 연구는 와송에 유기용매를 활용하여 순차적으로 추출하여 항염증 활성에 대한 가능성을 평가하기 하기 위해 수행되었다. 대식세포에 와송 hexane 추출물을 전처리하고 LPS로 염증을 자극하여 염증과 관련한 세포내 신호전달 경로에 미치는 영향을 확인하고자 하였다. 대식세포에 와송 hexane 추출물은 LPS 자극에 의해 세포 독성이 나타나지 않았고, ROS의 생성을 억제하는 것으로 확인되었다. 또한, IL-1β, IL-2, IL-6, IP-10과 같은 전염증성 사이토카인의 분비를 mRNA 수준에서 확인한 결과 탁월하게 억제하였다. 이러한 전염증성 사이토카인의 생성 억제는 상위 전사인자인 AP-1과 IRF3의 조절을 통해 이루어지므로 이들을 단백질 수준에서 발현량을 확인하였다. 그 결과 c-Jun, c-Fos, IRF-3의 인산화 억제로 핵 내 전사활성이 제한되었을 것으로 생각된다. 이들 결과를 종합해볼 때, 와송 hexane 추출물은 염증 반응을 저해하는 효과가 있는 것으로 나타나 다양한 염증성 질환의 예방 및 개선에 유용하게 활용할 수 있을 것으로 생각된다.

9송절편을 첨가한 송이주의 향기 성분

저자 : 윤정아 ( Jeong-ah Yoon ) , 신인웅 ( In-ung Shin ) , 박은희 ( Eun-hee Park ) , 이하연 ( Ha-yeon Lee ) , 김명동 ( Myoung-dong Kim )

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 48권 3호 발행 연도 : 2020 페이지 : pp. 316-321 (6 pages)

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송이버섯이 첨가된 전통주를 제조한 후 송절편을 첨가하여 첨가량과 숙성 기간에 따른 향기 성분의 변화를 기체 크로마토그래피 질량분광법을 통해 조사하였다. 송절편을 첨가하지 않은 대조구에서는 총 3종의 향기 성분이 검출되었으나, 송절편을 첨가한 송이주에서는 총 15종의 향기 성분이 검출되었다. 송절편을 5% 첨가한 실험구에서 송이버섯 특유의 향기 성분인 β-fenchyl alcohol과 2-octanol, methyl cinnamate은 숙성기간 동안 상대적인 함량이 증가하여 8주후에는 각각 67.57%, 2.14%와 0.94%를 나타냈다. 주성분 분석 결과 송절편 첨가에 의한 β-fenchyl alcohol의 상대적 함량 증가가 송이주의 향미 특성에 영향을 미치며, 송이주의 향미 특성은 송절편의 첨가량 보다 숙성 기간에 의해 더 큰 영향을 받는 것으로 판단된다.

10Identification of a Prophage-encoded Abortive Infection System in Levilactobacillus brevis

저자 : Marine Feyereisen , Jennifer Mahony , Tadhg O Sullivan , Viktor Boer , Douwe Van Sinderen

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 48권 3호 발행 연도 : 2020 페이지 : pp. 322-327 (6 pages)

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Abortive infection systems (Abi) are phage resistance systems that can be prophage-encoded. Here, two genes encoding an Abi system were identified on a prophage sequence contained by the chromosome of the Levilactobacillus brevis strain UCCLBBS124. This Abi system is similar to the two-component AbiL system encoded by Lactococcus lactis biovar. diacetylactis LD10-1. The UCCLBBS124 prophage-derived Abi system (designated here as AbiL124) was shown to exhibit specific activity against phages infecting L. brevis and L. lactis strains. Expression of the AbiL124 system was shown to cause reduction in the efficiency of plaquing and cell lysis delay for phages of both species.

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주제별 간행물
간행물명 수록권호

KCI등재 SCI SCOUPUS

Mycobiology
48권 5호 ~ 48권 5호

한국환경생태학회 학술발표논문집
2020권 1호 ~ 2020권 1호

KCI등재

한국환경생태학회지
34권 5호 ~ 34권 5호

KCI등재 SCI SCOUPUS

Journal of Microbiology and Biotechnology
30권 10호 ~ 30권 10호

CBF 바이오경제 이슈포커스
2020권 7호 ~ 2020권 7호

KCI등재

한국균학회지
48권 3호 ~ 48권 3호

KCI등재 SCOUPUS

식물분류학회지
50권 3호 ~ 50권 3호

KCI등재 SCOUPUS

한국미생물·생명공학회지
48권 3호 ~ 48권 3호

KCI등재 SCI SCOUPUS

Journal of Microbiology and Biotechnology
30권 9호 ~ 30권 9호

KCI등재

한국키틴키토산학회지
25권 3호 ~ 25권 3호

KCI등재 SCI SCOUPUS

Mycobiology
48권 4호 ~ 48권 4호

CBF 바이오경제 이슈포커스
2019권 3호 ~ 2020권 5호

KCI등재

한국환경생태학회지
34권 4호 ~ 34권 4호

CBF 바이오경제 이슈포커스
2019권 1호 ~ 2020권 6호

KCI등재 SCI SCOUPUS

Journal of Microbiology and Biotechnology
30권 8호 ~ 30권 8호

KCI등재 SCI SCOUPUS

Journal of Microbiology and Biotechnology
30권 7호 ~ 30권 7호

KCI등재 SCI SCOUPUS

Mycobiology
48권 3호 ~ 48권 3호

KCI등재

한국균학회지
48권 2호 ~ 48권 2호

KCI등재 SCOUPUS

식물분류학회지
50권 2호 ~ 50권 2호

KCI등재

한국키틴키토산학회지
25권 2호 ~ 25권 2호
발행기관 최신논문
자료제공: 네이버학술정보
발행기관 최신논문
자료제공: 네이버학술정보

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